Description

1. Introduction to Plant Tissue Culture
2. Scope of Plant Tissue Culture
3. Importance of Plant Tissue Culture
4. History of Plant Tissue Culture Technology
5. Tissue Culture Laboratory Organization
6. Tissue Culture Laboratory Requirements
7. Sterilization Techniques
8. Nutrient Media
9. Composition of Nutrient Medium
10. Preparation of Stock Solutions
11. Preparation of Culture Medium
12. Types and Methods of Plant Tissue Culture
13. Totipotency of Cells
14. Callus Culture
15. Apical Meristem Culture
16. Protoplast
17. Protoplast Culture
18. Isolation of Protoplasts
19. Protoplast Fusion
20. Selection of Hybrid Protoplasts
21. Regeneration of Hybrid Plants
22. Importance of Protoplast Fusion
23. Cybrid
24. Artificial Seeds
25. Production of Artificial Seeds
26. Introduction to Genetic Engineering
27. Techniques of Genetic Engineering
28. Construction of Recombinant DNA
29. Isolation of Specific Genes
30. Enzymes Used in Gene Cloning
31. Restriction Enzymes
32. Restriction Modification Systems
33. Gene Cloning Vectors
34. Plasmids
35. Properties of Plasmids
36. Plasmid Types
37. Properties of an Ideal Gene Cloning Vector
38. pBR 322
39. pUC8
40. Ti Plasmid
41. Disarmed Ti Plasmids
42. Bacteriophage Vectors
43. Lambda Phage Vector
44. M13 Bacteriophage Vector
45. Cosmids
46. Phagemids
47. Plant Viral Vector - CaMV of Cosmid
48. cDNA Libraries
49. Agrobacterium Mediated Gene Transfer in Plants
50. DNA Transfer Techniques
51. Microinjection
52. Calcium Phosphate- Chemical Methods of DNA Transfer (Transfection)
53. Electroporation (Electrical Method of DNA Transfer)
54. Bacterial Conjugation
55. Bacterial Transformation-Natural Method of DNA Transfer
56. Genetically Modified Plants (GM Plants)
57. Bt Brinjal
58. Golden Rice
59. Bt Cotton
60. Bioethics
61. Identification or Selection of Recombinants
62. Insertional Inactivation
63. Immunological Method
64. Colony Hybridization
65. Selection of Recombinants Using Reporter Genes
66. Blotting Techniques
67. Southern Blotting
68. Northern Blotting
69. Western Blotting
70. Mutagenesis
71. Site Directed Mutagenesis
72. Random Mutagenesis